Add a scale bar in Fiji

Prereqresite:

• Download and install Fiji
• Have an image with Metadata

• Whould be an.ome.tif,.nd2, or.dv

• Step 1: Open your Image in Fiji by first opening the Bioformats shortcut window

• Step2: Drag and drop your file on the shortcut window
  *Or you can open from Bio Format Importer

• Step3: Click ‘OK’ from the window below
  *‘Display OME XML metadata’ will show you the pixel size embedded in the image if you want to double-check that you have pixel sizes that are correct/make sense
  *‘Use virtual stack’ (red arrow) will help with opening up large-sized z stack images faster!

• Step 4: Optional check pixel size, this is an example image showing pixel size area with red arrows

• After checking the pixel size, you can add the scale bar from the menu
  Analyze > Tools > Scale bar
  *Please make a copy and put the scale bar on the copy of the image, since it can be an irreversible process.