Huygens

Huygens is an easy-to-use commercial deconvolution program. We have it installed on Deconvolution Workstation 3 and older version are on the other two Deconvolution Workstations.

Their documentation wiki is here; the username to access it is UCSF_NIC and the password is Objective4.

Pinhole Calculator link for the Nikon TE2000-E with C2 scanning head

CSU-22/CSU-X1 Back projected Pinhole radius and spacing distance  by magnification

Magnification	Radius	Spacing Distance
100X	250nm	2.53um
60X	416.7nm	4.22um
40X	625nm	6.22um
20X	1250nm	12.7um
10X	2500nm	12.7um

CSU-W1 Back projected Pinhole radius and spacing distance  by magnification

Magnification	Radius	Spacing Distance
100X	250nm	5um
60X	416.7nm	8.33um
40X	625nm	15um
20X	1250nm	30um
10X	2500nm	50um

CREST-V2 Back projected Pinhole radius and spacing distance  by magnification

Magnification	Radius	Spacing Distance
100X	250nm	2.5um
60X	416.7nm	4.16um
40X	625nm	6.25um
20X	1250nm	12.5um
10X	2500nm	25um