Spinning Disk Confocal

FPBASE

Optics

Objectives

1. Plan Fluor 10x /0.3 (Ph1 DL) -910 nm/pixel
2. Plan Apo 40x/0.95 Corr (DIC N2 / 40X I) - 227.5 nm/pixel
3. Plan Apo VC 60x/1.2 WI- 154nm/pixel
4. Plan Apo VC 60x/1.4 Oil -154 nm/pixel
5. Plan Apo VC 100x/1.4 Oil (DIC N2 / 100X I) 91 nm/pixel
6. Apo TIRF 100x/1.49 Oil 91 nm/pixel

Filter Turret 1 (FL1, Top)

1. Empty
2. DIC Analyzer
3. GFP (long pass)
4. YFP
5. Rhodamine
6. D/F/R

Yokogawa Dichroics

1. 405/491/561/640

Emission Wheel

(To get NIS-Elements numbering, add 1 to these numbers)

1. Open
2. ET460/50m
3. ET525/50m
4. ET645/65m
5. ET700/75m
6. ET610/60m
7. zET405/488/561/635m
8. Empty
9. Empty
10. Empty

Hardware

• Nikon Ti-E Microscope
• Yokogawa CSU-22
• Sutter Lambda LS-2 lamp for epifluorescence illumination
• Sola II LED (Lumencore)
• Sutter Emission wheel controlled by Lambda 10-B
• Sutter TLED brightfield illumination
• Photometrics Evolve Delta with 1.7X relay lens in front
• Prior motorized stage with Piezo Z-drive
• Nikon XY stage
• Mad City Lab Piezo Stage (100um)
• Okolab enclosure and Bold Line incubator
• NI PXI-1033 Controller; 5 Slot PXI Xchassis with Integrated MXIController

Lasers

• 405 nm, 100 mW Coherent OBIS
• 488 nm, 150 mW Coherent OBIS
• 561 nm, 100 mW Coherent OBIS
• 640 nm, 100 mW Coherent OBIS

Interface Requirements

• 1 serial port for Sutter Lambda 10-B (COM5)
• 1 serial port for Yokogawa CSU-22 (COM)
• 1 PCIe to highspeed serial port for Prior Proscan II (COM6)
• Firewire for Evolve
• USB for Ti
• USB for laser controller (COM4; MM only)

Firmware

• Ti: Combination K
• Ti-El: V5.2 Sum Fa24h
• TiPad: V5.1 Sum 08C4h
• TiHUBC/A Hub Controller A: V5.2 Sum C183h, V5.2 Sum 340Bh

PC

• Windows 11
• HP Z4
• Intel Xeon 3.6 GHzprocessor (4 cores)
• 32 GB RAM DDR4 2666 Mhz
• NVIDIA RTX 4000 8GB
• 512GBTurbo M.2 OS drive
• 240GB SSD
• 2x2TB HDD storage

Software

• Nikon Elements 5.02 build 1266
• Micro-Manager 2.0 gamma (how to cite Micro- Manager: https://micro-manager.org/Citing_Micro-Manager)