TruLive3D Light Sheet

Optics

Objectives

1. Two Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lens for illumination

2.  One Nikon CFI Apo 25x (eff. 50x) W 1.1 NA water immersion objective lens for
    detection

Tube lens Magnifier for detection objective

1. 0.5 x = Effective Magnification 12.5 x
2.  1 x  = Effective Magnification 25 x
3.  1.5 x  = Effective Magnification 37.5 x
4.  2 x   = Effective Magnification  50 x

Excitation Wavelengths:

• For light sheet
• 405nm
• 488nm
• 515nm
• 561nm
• 642nm
• 685nm
• For Ablation
• 532 Pulsed CryLas

Transmission Light

• 880 LED for Bright field

Emission Filter Short Camera

1. BP 418-462 = Chroma ET 440/40
2. LP 498 = Semrock 488LP
3. BP 497-554 = Semrock 525/50
4. BP 526-564 = Chroma ET 545/40
5. BP 580-627 = Chroma ET 605/50
6. BP 610-629 = Semrock 620/14

Emission Filter Long Camera

1. LP 498 = Semrock 488LP
2. BP 497-554 = Semrock 525/50
3. BP 526-564 = Chroma ET 545/40
4. BP 580-627 = Chroma ET 605/50
5. BP 610-629 = Semrock 620/14
6. BP 655-704  = Semrock Brightline 679/41
7. LP 656 =  Chroma 655 LP
8. LP 696 = Chroma 700LP
9. BP 710-749 = Semrock 730/39

Dichroics between cameras

1.  500LP Camera splitting dichroic

2. Splits emission light longer than 500 to one
    camera and shorter than 500nm to a second camera

3.  565LP Camera splitting dichroic

4.  Splits emission light longer than 565 to one
    camera and shorter than 565nm to a second camera.

5. 640LP Camera splitting dichroic
6.  Splits emission light longer than 640 to one
    camera and shorter than 640nm to a second camera.

📄 Dichroic Diagram

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Hardware

• Hamamatsu Fire sCmossCamera x2
•  MEMS-Based Steering Module for Advanced Beam Control and Destriping
•  Dispersion-free iris-based beam expander module (Tag lens)
•  Temperature control for the MuVi or InVi SPIM sample chamber with gas mixer (Ibidi) for full
   control of gas composition (CO2, O2, humidity). Range 18 – 40°C
• Currently set up for CO2 only
• Luxendo MuVi SPIM Laser Switcher

•  Switches visible lasers between the light sheet optics and
   photomanipulation module.

•  Ablation Photomanipulation unit for a MuVi SPIM

Lasers

• 405 nm CW diode laser, 40mW before fiber
• 488 nm CW diode laser, 40mW before fiber
• 515 nm CW diode laser, 40mW before fiber
• 561 nm CW diode laser, 40mW before fiber
• 642 nm CW diode laser, 40mW before fiber
• 685 nm CW diode laser, 40mW before fiber
• Pulsed 532nm VIS laser, 1.5ns pulse length
   Diffraction-limited focal size

Neutral density filters for lasers

• 1%
• 10%
• 100%

PC

• Win pro 11 Verxion 23H2
• AMD Thred Ripper Pro 5955X 16-Cored 4.0 Ghz
• 256 RAM
• C: Drive (SSD) 1TB
• Data Drive 30TB, micron MTFDKCC30T7TGH-1BC1ZABYY, R/W speed 7000 MB/s

Software

• Lux control 5.01

Data Handling

The Data is big! make sure to get on our server, visit the Data Storage and Compute page

Python scripts page for Luxendo

Luxendo Image Git Hub

Read here for the Data structure and opening:

Documentation

Manual

📄 TruLive Manual

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Photo Manipulation

📄 Photomanipulation

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📄 Luxendo Photomanipulation

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📄 CryLas Photomanipulation

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