UCSF CALM Microscopy Wiki
UCSF CALM Microscopy Wiki
Wiki
View the Project on GitHub
UCSF-CALM/wiki
Contents:
- Home
- Microscopes
- Spinning Disk Confocal
- TIRF/N-STORM
- Time Lapse
- 6D
- Crest LFOV/C2
- CSU-W1
- AZ100 Light Sheet
- OMX-SR
- TruLive3D Light Sheet
- C-Trap Optical Tweezers
- QLIPP Timelapse with Nanoindentor
- Snouty Light Sheet
- CVRI Nikon Spinning Disk
- CVRI Nikon Spinning Disk
- CVRI Leica SPE Confocal
- Other Equipment and Objectives
- Data Analysis Workstations
- Data Analysis
- Sample Preparation
- References & Education
- CALM Information
- Miscellaneous
Contents:
- Home
- Microscopes
- Spinning Disk Confocal
- TIRF/N-STORM
- Time Lapse
- 6D
- Crest LFOV/C2
- CSU-W1
- AZ100 Light Sheet
- OMX-SR
- TruLive3D Light Sheet
- C-Trap Optical Tweezers
- QLIPP Timelapse with Nanoindentor
- Snouty Light Sheet
- CVRI Nikon Spinning Disk
- CVRI Nikon Spinning Disk
- CVRI Leica SPE Confocal
- Other Equipment and Objectives
- Data Analysis Workstations
- Data Analysis
- Sample Preparation
- References & Education
- CALM Information
- Miscellaneous
AZ100 Light Sheet
This microscope is being retired. After Many years of Service the AZ100 no longer wishes to behave.
FPBASE
Notes on Usage
-
Make sure your sample is centered in the cuvette. Imaging close to the edges of the cuvette often induces aberrations that result in poor image quality.
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In general, the 5mm slit provides the best balance of light sheet width and taper. The smaller slits result in thicker light sheets with little benefit, unless you are using the 1x objective with a low zoom.
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Don't use zooms less than 2x unless absolutely necessary. There are substantial aberrations at the edges of field of view at low zooms.
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At 3x zoom and below, set the CameraCrop parameter to 2048 x 2048 - this eliminates aberrations present at the edges of the field of view at these lower magnifications.
-
With the 5x objective, you may need to adjust the AZ100 Z position in steps as small as 2 um to get an accurate focus.
Az100 Cuvets:
Cuvette Images
Az100 Cuvetts
Az100-cuvets on scope
Click Below to expand or download detailed information on Cuvets
📄 AZ100 Large Cuvette
📄 Light Sheet Custom Cuvette
Documentation
📄 AZ100 Characterization
Kurt’s Blog - Building the Microscope
📄 Building a Lightsheet Microscope Around an AZ100, Part 1
📄 Building a Lightsheet Microscope Around an AZ100, Part 1 (Comments)
📄 Building a Light Sheet Microscope Around an AZ100, Part 2
📄 Building a Light Sheet Microscope Around an AZ100, Part 2 (Comments)
📄 Kurt's Blog - AZ100 Part 1
📄 Kurt's Blog - AZ100 Part 2a
Image Processing- Destriping Plugin
📄 VSNR Documentation (Fiji Plugin)
📄 VSNR IEEE Paper
Optics
Objectives
| Position | Objective | Pixel Size |
|---|---|---|
| 1. | AZ Plan Fluor 2x | |
| 2. | AZ Plan Fluor 5x | |
| 3. | AZ Plan Apo 1x |
Emission Wheel
- Position 0: Open
- Position 1: ET455/50m
- Position 2: ET535/36m
- Position 3: ET605/52m
- Position 4: ET705/72m
- Position 5: Open
- Position 6: Closed
- Position 7: Closed
Turret (Manual for EPI)
- Empty
- 96362 ET GFP C94925
- 96365 ET TXR C93169
- 96345 HQ:Y GFP C77392 (from C1)
Hardware
- Nikon AZ100
- Vortran 4-line Laser Launch
- Andor Zyla 5.5 Camera
- Custom light sheet optics
Lasers
- 405nm 100mW
- 488nm 50mW
- 561nm 50mW
- 640nm 110mW
Interface
- 1 USB Arduino-Hub (COM5, baud Rate 57600)
- ASI MFC-2000 Z stage (COM1, baud rate 9600)
- 1 Camera card slot-Zyla 5.5
- 1 serial port ASI Controller, XYZ Stage, Camera stage (COM3 baud rate 115200)
- COM2 baud rate 115200
- 1 USB for Vortan
- 1 USB for Az100
PC
- Core i7 4770K (4 Ghz)
- 32 GB RAM
- NVidia GTX970
- Windows 10 Enterprise 64bit
- 250GB SSD
- 2TB Data drive