CVRI Nikon Spinning Disk Confocal # 2

FPBASE

Optics

Objectives

Position	Objective	Pixel Size
1.	10X λ Plan Apo 0.45 NA
2.	20x Plan Apo VC 0.75NA DIC N2
3.	40x Plan Apo 1.3 NA (in cabinet)
4.	40x Plan Apo 0.95 NA (in cabinet)
5.	60x Plan Apo VC 1.4NA DIC H N2
6.	Plan Apo VC 100x 1.4 NA DIC 2 (in cabinet)

Filter Turret 1 (FL1, Top)

• EPI
• ZET 405/488/561/640x

Yokogawa Dichroics

1. 405/491/561/640

Emission Wheel Left Confocal

• FF01-609/57
• ET460/50m
• ET525/50m
• ET630/75m
• ET690/50m

Emission Wheel Right EPI

-

Hardware

• Nikon Ti Microscope (Inverted)
• Yokogawa CSU-X1 Spinning Disk Confocal (manual shutter)
• Vortran 4-line laser launch
• Lumincore LED
• ASI motorized XY stage (COM3, Baud Rate Auto) with Z piezo
• OkoLab Cage Incubator
• OkoLab CO2 Mixer
• Sutter FLED Dual-channel system with 440nm and 630nm LED (BF lightpath, NIDAQ)

Lasers

Vortran laser launch

• 405
• 488
• 561
• 647

PC

-

Software

• MicroManager2.0
• how to cite Micro Manager: https://micro-manager.org/Citing_Micro-Manager