CVRI Nikon Spinning Disk Confocal # 2

FPBASE

Optics

Objectives

• 10X λ Plan Apo 0.45 NA
• 20x Plan Apo VC 0.75NA DIC N2
• 40x Plan Apo 1.3 NA (in cabinet)
• 40x Plan Apo 0.95 NA (in cabinet)
• 60x Plan Apo VC 1.4NA DIC H N2
• Plan Apo VC 100x 1.4 NA DIC 2 (in cabinet)

Filter Turret 1 (FL1, Top)

• EPI
• ZET 405/488/561/640x

Yokogawa Dichroics

1. 405/491/561/640

Emission Wheel Left Confocal

• FF01-609/57
• ET460/50m
• ET525/50m
• ET630/75m
• ET690/50m

Emission Wheel Right EPI

-

Hardware

• Nikon Ti Microscope (Inverted)
• Yokogawa CSU-X1 Spinning Disk Confocal (manual shutter)
• Vortran 4-line laser launch
• Lumincore LED
• ASI motorized XY stage (COM3, Baud Rate Auto) with Z piezo
• OkoLab Cage Incubator
• OkoLab CO2 Mixer
• Sutter FLED Dual-channel system with 440nm and 630nm LED (BF lightpath, NIDAQ)

Lasers

Vortran laser launch

• 405
• 488
• 561
• 647

PC

-

Software

• MicroManager2.0
• how to cite Micro Manager: https://micro-manager.org/Citing_Micro-Manager