UCSF CALM Microscopy Wiki
UCSF CALM Microscopy Wiki
Wiki
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UCSF-CALM/wiki
Contents:
- Home
- Microscopes
- Spinning Disk Confocal
- TIRF/N-STORM
- Time Lapse
- 6D
- Crest LFOV/C2
- CSU-W1
- AZ100 Light Sheet
- OMX-SR
- TruLive3D Light Sheet
- C-Trap Optical Tweezers
- QLIPP Timelapse with Nanoindentor
- Snouty Light Sheet
- CVRI Nikon Spinning Disk
- CVRI Nikon Spinning Disk
- CVRI Leica SPE Confocal
- Other Equipment and Objectives
- Data Analysis Workstations
- Data Analysis
- Sample Preparation
- References & Education
- CALM Information
- Miscellaneous
Contents:
- Home
- Microscopes
- Spinning Disk Confocal
- TIRF/N-STORM
- Time Lapse
- 6D
- Crest LFOV/C2
- CSU-W1
- AZ100 Light Sheet
- OMX-SR
- TruLive3D Light Sheet
- C-Trap Optical Tweezers
- QLIPP Timelapse with Nanoindentor
- Snouty Light Sheet
- CVRI Nikon Spinning Disk
- CVRI Nikon Spinning Disk
- CVRI Leica SPE Confocal
- Other Equipment and Objectives
- Data Analysis Workstations
- Data Analysis
- Sample Preparation
- References & Education
- CALM Information
- Miscellaneous
Time Lapse
FPBASE
Optics
Objectives
- Plan Fluor 10x/0.3 (Ph1) 654nm/pixel
- Plan Apo 20x/0.75 (DIC N2 / 20X) 327nm/pixel
- Plan Fluor 40x/0.75 (DIC N2 / 40X I) 164nm/pixel
- Plan Fluor 40x/1.3 Oil (DIC N2/ 40X II) 164nm/pixel
- Plan Apo 60x/1.2 WI (DIC N2/ 60X II) 109nm/pixel
- Plan Apo 100x/1.4 Oil (Ph3) 65.4nm/pixel
Filter Turret
- Semrock DAPI/FITC/TRITC/Cy5 Sedat Quad
- GFP/mRFP1
- CFP/YFP (86002bs Chroma)
- Analyzer
- Empty
- FURA2 (Emission Filter within Cube)
Excitation from Lambda 821
| LED wavelength | Filter in front of LED |
|---|---|
| 340 nm | FF01-340/26-25-STR |
| 380 nm | FF01-389/38-25-STR |
| 440 nm | FF01-445/40-25-STR |
| 480 nm | FF01-480/40-25-STR |
| 530 nm | FF01-504/12-STR |
| 561 nm | FF01-562/40-25-STR |
| 630 nm | FF01-630/38-25-STR |
Emission Wheel
- Fura2 emission filter
- Empty
- 440/11m Semrock Sedat Quad
- 525/30m Semrock Sedat Quad
- 607/36m Semrock Sedat Quad
- 684/24m Semrock Sedat Quad
- ET525/50m GFP/mCherry ET (89021 Em2)
- ET632/60m GFP/mCherry ET (89021 Em1)
- ET470/24m CFP/YFP (89002 Em2)
- ET535/30m CFP/YFP (89002 Em1)
Hardware
- Nikon Ti-E Microscope
- External Phase Unit
- Nikon motorized stage
- Sutter Lambda 421 LED light source with NIDAQ interface
-
Sutter Emission wheel (wheel C) controlled by Lambda 10-B (COM7, 128k Baud Rate)
- Hamamatsu Flash 4.0 (Right port)
- OkoLab incubator and CO2 control
- QLIPP components above condenser
- Semrock Brightline FF01-540/50-25
- ThorLabs LPVISA100, Linear Polarizer, 480-550 nm
- Meadowlark LCs in 3D printed mount\
- 1st LC oriented 45 deg relative to the long edge of the housing
- 2nd LC oriented parallel to the long edge of the housing
Interface Requirements
- 1 serial port for 10-3
- 3 USB connectors
- 10-B COM7 (USB)
- NI PCI 6723 board
Firmware
- Ti: Combination K
- TiControl: 5.50
PC
- NIC-HighSpeed2
- i9-10900X 3.70GHz
- 32 GB RAM
- Windows 10 Enterprise 64bit
- NVIDIA T660 (4GB)
- 894GB SSD
- 5.46TB HDD
- Active Silicon FireBird Card (currently not in use for the Hammamstsu camera)
Software
NIS-Elements 5.21.03 (Build 1489)- MicroManager 2.0 (how to cite Micro- Manager: https://micro-manager.org/Citing_Micro-Manager)
Excitation Wheel (filters if you used Lambda XL lamp for earlier experiments)
- ET340x Fura-2 (79000 Ex1)
- ET380x Fura-2 (79000 Ex2)
- 387/11x Semrock Sedat Quad
- 485/20x Semrock Sedat Quad
- 560/25x Semrock Sedat Quad
- 650/13x Semrock Sedat Quad
- ET470/40x GFP/mCherry ET (89021 Ex2)
- ET572/35x GFP/mCherry ET (89021 Ex1)
- ET430/24x CFP/YFP (89002 Ex1)
- ET500/20x CFP/YFP (89002 Ex2)