C-Trap Optical Tweezers

About

The C-Trap is an easy to use optical trap made by  Lumicks.  Our current configuration has Microfluidics with two laser traps and confocal imaging. Specifics below:

Objectives

Nikon CFI Plan Apo 60 X/1.2 NA WI 270 WD

Condenser

Leica P, 60x, 1.40NA Oil 1.4NA WD

Lasers

Trapping laser: 1,064nm (20W at source)

Confocal Laser: 561nm only

Traps

One Dynamic Trap

One static

Micro Fluidics

Automatic Fluidics Control (AFL)

5 fluidic channels + Waste (6 total)

microfluidics

Data Analysis

For C-Trap data analysis instructions, see C-Trap Data Analysis

Manuals

Lumicks Manuals and documentation are only allowed to be shared internally per Lumicks guidlines. Please email the Core if you need access.

Troubleshooting Tips:

Trapping laser won't turn on

• If the system has not been used in a few days or more than the lasers may need some time to warm up!
• Tip: always turn the key first while you set up to give them as much time as possible
• Wait an extra few minutes (5-10min) if you did not turn it on first thing.
• Do a full restart

Condenser won't move

• It is likely stuck in a state. The whole system needs to be restarted.

Whole system restart

• Lower the Objective
• Turn off the system computer
• Turn off software first. Save anything you do not want to get lost.
• Turn off from  Windows Start menu
• Bridge computer can stay on
• Turn off Trapping laser
• Turn Key
• Turn off Main power Supply
• WAIT 3 min (set a timer if needed)
• Turn on main power supply
• A high-pitched noise is normal as a few of the components will reset to default "home positions"
• Turn on the system computer
• This is the box below the main power supply,
• There is a door with a key, you need to open the door then flip the switch
• Turn on the trapping  laser