6D

FPBASE

Optics

Objectives

Plan Apo 4x/0.2 - 1,834 nm/pixel
Plan Apo 10x/0.45 - 728 nm/pixel
Plan Apo 20x/0.75 - 365 nm/pixel
Plan Apo 40x/0.95 Corr - 183 nm/pixel
Plan Apo VC 60x/1.4 Oil - 122 nm/pixel
Plan Apo VC 100x/1.4 Oil (DIC N2 / 100X I) - 65.4nm/pixel

(other objectives)

Plan Apo 2x/0.1 - 3,270 nm/pixel
Plan Fluor 10x/0.3 - 728 nm/pixel
Super Fluor 40x/1.3 oil - 183 nm/pixel
Super Fluor 100x/0.5-1.3 iris - 65.4nm/pixel

Filter Turret

Analyzer
Empty
Empty
CFP/YFP (89002)
GFP/mCherry ET (89021)
Semrock 5-band FF408/504/581/667/762 (DA/FI/TR/Cy5/Cy7-5X5M-B-000)

Excitation Wheel

387/11x Semrock 5-band
485/20x Semrock 5-band
560/25x Semrock 5-band
650/13x Semrock 5-band
740/13x Semrock 5-band
ET470/40x GFP/mCherry ET (89021 Ex2)
ET572/35x GFP/mCherry ET (89021 Ex1)
ET430/24x CFP/YFP (89002 Ex1)
ET500/20x CFP/YFP (89002 Ex2)
Open

Emission Wheel

Open
440/40m Semrock 5-band
525/30m Semrock 5-band
607/36m Semrock 5-band
684/24m Semrock 5-band
809/81m Semrock 5-band
ET525/50m GFP/mCherry ET (89021 Em2)
ET632/60m GFP/mCherry ET (89021 Em1)
ET470/24m CFP/YFP (89002 Em2)
ET535/30m CFP/YFP (89000 Em1)

Hardware

Nikon Ti-E Microscope
Sutter Lambda LS lamp with filter wheel and shutter
Sutter Lambda 10-3 (COM 4)
Sutter Emission wheel and brightfield shutter
Nikon DS-Qi2 (fluorescence)
Nikon DS-Ri2 (color)

Interface Requirements

1 x USB for Nikon TI-E
1x USB for DS-Qi2 Camera
1 X USB DS-Ri2 Camera
1x USB Sutter Lambda 10-3

Firmware

TI-E: v5.2

PC

Core i9 9900XK (3.5 Ghz)
128 GB RAM
NVidia Quadro P4000 Pro 8GB
Windows 10 Enterprise 64bit
500 GB SSD
6TB HD
1TB SSD
Software
NIS elements 5.21.03 [b1489]