UCSF CALM Microscopy Wiki
UCSF CALM Microscopy Wiki
Wiki
View the Project on GitHub
UCSF-CALM/wiki
Contents:
- Home
- Microscopes
- Spinning Disk Confocal
- TIRF/N-STORM
- Time Lapse
- 6D
- Crest LFOV/C2
- CSU-W1
- AZ100 Light Sheet
- OMX-SR
- TruLive3D Light Sheet
- C-Trap Optical Tweezers
- QLIPP Timelapse with Nanoindentor
- Snouty Light Sheet
- CVRI Nikon Spinning Disk
- CVRI Nikon Spinning Disk
- CVRI Leica SPE Confocal
- Other Equipment and Objectives
- Data Analysis Workstations
- Data Analysis
- Sample Preparation
- References & Education
- CALM Information
- Miscellaneous
Contents:
- Home
- Microscopes
- Spinning Disk Confocal
- TIRF/N-STORM
- Time Lapse
- 6D
- Crest LFOV/C2
- CSU-W1
- AZ100 Light Sheet
- OMX-SR
- TruLive3D Light Sheet
- C-Trap Optical Tweezers
- QLIPP Timelapse with Nanoindentor
- Snouty Light Sheet
- CVRI Nikon Spinning Disk
- CVRI Nikon Spinning Disk
- CVRI Leica SPE Confocal
- Other Equipment and Objectives
- Data Analysis Workstations
- Data Analysis
- Sample Preparation
- References & Education
- CALM Information
- Miscellaneous
TIRF/N-STORM
FPBASE
Optics
Objectives
- Plan Fluor 10x /0.3 (Ph1) 1600 nm/pixel
- Plan Apo 20x/0.75 (DIC N2 / 20X) 785 nm/pixel
- Empty
- Plan Apo VC 60XA/1.20 Water
- Plan Apo lambda 100x/1.45 TIRF Oil 157 nm/pixel
- Apo TIRF 100x/1.49 Oil (DIC N2 / 100X I) 157 nm/pixel
Filter Turret 1 (FL1)
- Empty
- Analyzer
- Quad N-STORM
- DAPI/FITC/Rhodamine Triple (for eyepiece viewing with arc lamp)
- CFPHQ
- DAPI
Filter Turret 1 (FL2)
-
FRAP cube-Longpass dichroic for reflecting 473 and 405 to sample plane (T495lpxr-UF2)
- Empty
- Empty
- Empty
- Empty
- Empty
FRAP unit v
Emission Wheel (Andor Du897)
- ET455/50m
- ET525/50m
- ET600/60m
- ET705/72m
- ZET488/561/640m (triple bandpass)
- DualBP 59012m (515/30m, 598/45m)
- ZET405/488m-TRF
- Empty
- Blocker
- Blocker
Emission Wheel (Hamamatsu)
- Blocker
- ZET405/488/561/640m (quadruple bandpass)
- ET455/50m
- ET525/50m
- ET600/60m
- ET705/72m
- Empty
- Empy
- Empty
- Empty
Hardware
- Nikon Ti-E Microscope
Sutter emission wheel and 10-B (COM 5 baud rate auto)- Sutter Lambda 10-3 Controller
- Nikon motorized stage
- Andor DU897 on right port
with 3D STORM lens - Hammamatsu Orca Flash 4 (left port, COM 4)
- Sutter LB 10-N filter Wheel in front of each camera
- Agilent MLC400 (405nm, 488nm, 561nm, 647nm) with NIDAQ interface(v18.oo)
- Brightfield shutter controlled by Sutter Lambda SC through Ti
- FRAP System UGA-42 Firefly
- FRAP lasers diode lasers - 405nm /100mW and 473nm 100mW
Interface Requirements
- Camera link board- Orca Flash 4 PCIe- 1x-CLD-2PE8
- CCI-24- PCIe controller card-Andor DU897
- NI PCI 6723 board connected though PXI box -Agilent Lasers MLC 400B
- Custom board added for FRAPintegration
- USB for Sutter 10-B
- USB Oko lab bold line
- USB - Nikon Ti
Firmware
- Ti: Combination I
- TiControl: 5.2
PC
- HP Z4
- Intel Xeon 3.7 GHzprocessor (8 cores),
- 64GB DDR4 2666 Mhz RA
- NVIDIA RTX4000 8GB
- 512GBM.2 Turbo SSD O/S drive
- 1TB M.2 Turbo SSD data drive
- 12TB datastorage
Software
- MicroManager 2.0
- how to cite Micro Manager: https://micro-manager.org/Citing_Micro-Manager