Crest LFOV Spinning Disk/ C2 Confocal

FPBASE

Optics

Objectives

Position	Objective	Pixel Size
1.	CFI Plan Apo Lambda 10x /0.45	1,132 nm/pixel
2.	CFI Plan Apo Lambda 20x/0.75	567 nm/pixel
3.	CFI Plan Apo Lambda 40x/0.95 Corr	285 nm/pixel
4.	CFI Plan Apo Lambda S 40x/1.25 Corr (Silicone)	285 nm/pixel
5.	CFI Plan Apo Lambda 60x/1.4 (Oil)	187 nm/pixel
6.	CFI Pla Apo VC 100x/1.4 (Oil)	114 nm/pixel

Filter Turret 1

1. DAPI
2. GFP
3. Texas Red
4. Empty
5. Empty
6. 405nm Reflect/430-800nm Trans Dual TIRF (FRAP)

Crest Dichroics

1. 405/488/561/640/750
2. 440/540

Emission Wheel (Crest)

1. Nikon Multiband
2. FF01-438/24
3. FF01-485/20
4. FF01-511/20
5. FF01-595/31
6. FF01-560/25
7. FF02-685/40
8. FF01-819/44

Hardware

• Nikon Ti2-E Microscope
• Crest X-Light V2 L-FOV spinning disk confocal
• Nikon C2 confocal with DUVb detector
• MCL piezo stage
• Opti-Microscan FRAP with 405nm laser line
• Lumencor Sola SE U-NIR
• Lumencor Celesta Light engine
• Prime 95B 25mm sCMOS camera
• Okolab enclosure and Bold Line incubator

Lasers

C2 Confocal

• 405 nm
• 488 nm
• 561 nm
• 640 nm

Crest Confocal (Lumencor Celeste Light engine)

• 405 nm
• 446 nm
• 477 nm
• 520 nm
• 546 nm
• 638 nm
• 749 nm

Interface Requirements

Firmware

PC

• Windows 10 Pro 64-bit
• Core i7 3.4GHz
• 16 GB RAM
• NVidia GTX550Ti
• 240GB SSD
• 2TB Hard drive (RAID 1)

Software

• Nikon Elements 5.41.01 build 1709