CVRI Nikon Spinning Disk Confocal # 1

FPBASE

Optics

Objectives

• 10X λ Plan Apo 0.45 NA
• 20x Plan Apo VC 0.75NA DIC N2
• 40x Plan Apo 1.3 NA (in cabinet)
• 40x Plan Apo 0.95 NA (in cabinet)
• 60x Plan Apo VC 1.4NA DIC H N2
• Plan Apo VC 1.4 NA DIC 2 (in cabinet)
• 100x Apo TIRF 100x Oil DIC N2 (in cabinet)

Filter Turret 1 (FL1, Top)

• analyzer
• UV2E/C
• TRITC- Chroma 41002c
• ZET 405/488/561/640x
• FITC

Yokogawa Dichroics

1. 405/491/561/640

Emission Wheel Left Confocal

• FF01-609/57
• ET460/50m
• ET525/50m
• ET630/75m
• ET690/50m

Emission Wheel Right

• 690/50m
• 630/75m
• 525/50m

Hardware

• Nikon Ti Microscope (Inverted)
• Yokogawa CSU-X1 Spinning Disk Confocal
• Vortran 4-line laser launch (connected via NIDAQ PXI box)
• Sutter Lambda XL (COM6)
• Photometrics Prime95B sCMOS camera (left port, USB)
• Andor DU897 EMCCD, (Right port, CCI-24 PCIe Controller Card)
• Sutter Lambda 10-3 emission filter wheel: Epi-Right, Confocal-Left (COM4)
• ASI motorized XY stage (COM3, Baud Rate Auto) with Z piezo
• OkoLab Cage Incubator
• OkoLab CO2 Mixer
• Sutter FLED Dual-channel system with 440nm and 630nm LED (BF lightpath, NIDAQ)

Lasers

Vortran laser launch

• 405
• 488
• 561
• 647

PC

• Windows 10 Pro 64-bit
• ASUS Prime X299-A
• Core i7 7740x (4.3 GHz)
• 64GB Memory
• NVIDIA GTX1060
• 512 GB M.2
• 1 TB SSD RAID 0
• 8 TB HD

Software

• Nikon Elements 5.02 build 1266
• MicroManager 2.0 (how to cite Micro- Manager: https://micro-manager.org/Citing_Micro-Manager)