User Quick Start Guides

Microscopy References and Education

General Micromanager Protocols

📄 Using the stage position List in MM The stage position allows for multi point imaging and the grid creator lets you tile!

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General NIS-Elements Information

📄 What is Changing: Apperance Vs. Data in Elements The stage position allows for multi point imaging and the grid creator lets you tile!

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📄 Shading Correction Fluorescence in Elements The stage position allows for multi point imaging and the grid creator lets you tile!

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Nikon Ti Guides

📄 Key Buttons on Nikon Ti Base

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CSU-22 (Spinning Disk)

📄 No Red Channel (561nm) laser

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Color and Bright Field Imaging Quick Guides

Note: These are geared towards bright field color imaging on the 6D microscope running NIS Elements 5.21. The steps for Kohler will be the same on any microscope.

📄 Setting up kohler

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📄 Auto White for Color Imaging in Elements

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📄 Shading Correction for Color Imaging in Elements

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📄 Shading Correction for Fluorescence Imaging in Elements

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📄 Taking a Stitched Large Image Using JOBS in Elements

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📄 Export Stitched Images from Elements

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6D Widefield High Throughput

📄 Setting Up 6D and Focusing on Sample

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CSU-W1 Spinning Disk

📄 FRAP Set up W1

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C2/CREST Spinning Disk LOV

📄 Setting up Spinning Disk Channels on CREST

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CVRI Spinning Disk 1

📄 CVRI SD #1 Startup and Shutdown

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📄 CVRI SD #1 Turn on and OFF CO2 Pump

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📄 CVRI SD #1 Elements Set up

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CVRI Spinning Disk 2

📄 CVRI SD2 Startup and Shutdown

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📄 CVRI SD2 Startup Micro-Manager Info

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📄 CVRI SD2 Protocol Visual

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📄 Positions of CVRI SD#2 Manual Turrets fo Epi (eyepiece) and Confocal (lasers)

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📄 Rotating the image on CVRI SD #2 for the Image J Image Stitcher

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