CVRI Leica SPE Confocal

This microscope is being retired because the lasers do not turn on reliably.

Optics

Objectives

Position	Objective	Pixel Size
1.	ACS APO 10x/0.3
2.	HCX APO LUVI 20x/0.5 WI
3.	ACS APO 40x/1.15 Oil
4.	ACS APO 63x/1.3 Oil

Emission:

• SP detector 430-750nm

Hardware

• Upright Leica TCS-SPE-II DM5500 RYBV (loaner Leica DM600 B)
• PMT detector for fluorescence (capable of 8 or 12bit)
• PMT detector for transmission (capable of 8 or 12bit)
• Leica solid state laser launch
• Leica motorized stage
• EL6000 fluorescence lamp

Lasers

• 405nm
• 488nm
• 561nm
• 630nm

PC

• Windows 7 Pro 64-bit Windows Extenstion
• HP Z440 Workstation
• Xenon E5-1620 v3
• Quadro K620
• 8GB RAM

Firmware

• V02.3.7205
• Condenser: V01.03
• CTR: V01.5.4643

Software

• LAS AF