QLIPP-Timelapse with Nanoindentor

Optics

Objectives

• PLAN APO λ 10X/0.45 NA
• PLAN FLUOR Ph1 10x/0.3 NA
• PLAN APO 40/X.0.95 NA
• Other objectives available in cabinet

Filter Turret

1. Analyzer
2. QLIPP
3. 89000 - Sedat Quad - ET
   • 425 to 466 nm
   • 503 to 542 nm
   • 571 to 628 nm
   • 661 to 728 nm
4. Empty
5. 89006 - CFP/YFP/mCherry - ET
   • 454 to 486 nm
   • 518 to 559 nm
   • 597 to 667 nm
6. 89006 - CFP/YFP/mCherry -ET
   • 454 to 486 nm
   • 518 to 559 nm
   • 597 to 667 nm

Excitation Wavelengths Spectra X

1. 395 nm
2. 440 nm
3. 470 nm
4. 508 nm
5. 555 nm
6. 640 nm

Emission Wheel (Sutter)

1. Empty
2. 89000 - Sedat Quad - ET (Em3) - 430 to 480
3. 89000 - Sedat Quad - ET (Em2) - 507 to 546
4. 89000 - Sedat Quad - ET (Em1) -  579 to 631
5. 89000 - Sedat Quad - ET (Em4) - 669 to 741
6. 89006 - CFP/YFP/mCherry - ET (Em3) - 458 to 482
7. 89006 - CFP/YFP/mCherry - ET (Em1) - 520 to 550
8. 89006 - CFP/YFP/mCherry - ET (Em2) - 602 to 662

Hardware

• Nikon Ti-E Microscope
• Sutter Lambda LS lamp with filter wheel and shutter
• Sutter Lambda 10-3 (COM 4)
• Sutter Emission wheel
• Sutter FLED (BF LED)
• Zyla 4.2 Camera Link
• National Instrument BNC-2110
• Lumencor Spectra X (COM3)
• Triggerscope
• MCL piezo
• Oko Box with temperature control
• For QLIPP
• Medowlark Optics Liquid Crystal digital interface
• Chiaro Nanoindenter from Optics11 Life

Interface Requirements

•  Spectra X - RS232
• Zyla 4.2 - PCIe Camera link Card

Firmware

• Ti - E V 5.2

PC

• Intel(R) Core(TM) i7 CPU  870  @ 2.93GHz   2.93 GHz
• 16GB RAM
• ATI Radeon HD 4550
• 64-bit operating system, x64-based processor
• Windows 10 Pro
• 1TB C drive
• 3.5TB Data drive (SATA)

Software

• NIS Elements 5.21.01 build 1483
• NIDAQ  (v21.3.0)

Information on QLIPP

Collaboration required for QLIPP please contact the Core

https://pubmed.ncbi.nlm.nih.gov/32716843/

User Manuals & Documentation

📄 Chiaro User Manual v3.4

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📄 DataViewer User Manual V1.3

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Quick Start Guides

📄 Quick Startup of Indentation - Chiaro V4

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📄 Guidelines for Indentation (Chapter 2)

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Calibration & Setup

📄 Live Calibration v1.1

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📄 Probe Calibration V1

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📄 Find Surface (Old Hardware)

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Probes & Tips

📄 Probes Information (2023-05-04)

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📄 Tip Selection Guidelines v1.3

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📄 MEMS Information (V1 0-25 to 0-5 and 3-5)

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Sample Preparation

📄 Sample Preparation V4

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📄 Organoid Preparation

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Maintenance

📄 Cleaning Protocol v1.3

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