Sample Preparation

If you have used something on our microscopes that worked well (or didn’t!) please let us know! We would love to add it to this section for general knowledge.

Fluorescent Protein Information

• FPbase — Great resource for Fluorescent protein information
  • Interactive Chart of Fluorescent Proteins
  • Spectra viewer
• Fluorescent Proteins for Localization Microscopy

Reporter Proteins

• ATP/ADP PercevalHR

Dyes

• Dyes for 405nm excitation
• Mitochondrial Dyes
• Cy7 and 750nm Range Dyes

Methods and Protocols

• Clearing methods
• Sample preparation for SIM imaging
• STORM sample preparation and imaging
• Protocols for Immunocytochemistry in cell culture
• Calcium-FURA2 imaging
• Brain slice Preparation
• Cytoskeleton fixation references

Consumables Information/Materials

• Plates and dishes for imaging
• High Precision Coverslips
• Make your own flow chamber