Fiji- Viewing orthogonal slices

  1. Orthogonal slices allow you to view your z stacks from the slide!
  2. In Fiji OPen your image
  3. First set the colors and brightness and contrast for each image
  4. Color → on Channel on channel bar → go to LUT button and choose channel
  5. Brightness contrast- Image → Adjust → Brightness and Contrast Brightness and Contrast settings
  6. Image→Stacks→ Orthogonal views Orthogonal views menu
  7. Move around the cross hairs to see the different regions of the z sections Orthogonal view result