Finding your pixel size

As long as you have your raw data and it was taken in the CALM it should be a.ome.tif,.nd2, or.dv file. These steps below will also work with any file as long as the metadata has properly been recorded.

Prerequisite: Download and open fiji (https://fiji.sc/)

• Step1: Open the Bio-Format Plugins Shortcut Window

• Step2: Drag and drop your file on the shortcut window
  *Or you can open from Bio-Format Importer

• Step3: Click ‘OK’ from the window below
  *‘Display OME XML metadata’ will show you the pixel
  size embedded in the image
  *‘Use virtual stack’ (red arrow) will help with opening
  up large sized z stack images faster!

• In the OME Metadata window
• You can double check the pixel size from this window (red arrow)
  *This is just an example screenshot