CVRI Nikon Epifluorescence Microscope

This microscope is no longer Operational! This page is being left up for historical information.

This microscope has been decommissioned.

FPBASE

Optics

Objectives

Position	Objective	Pixel Size
1.	Plan Apo 4x/0.2
2.	Plan Fluor 10x/0.3
3.	Plan Apo VC 0.75NA 20x
4.	Plan Apo 20X/0.45 ELWD
5.	Plan Apo VC 60x/1.4

Filter Turret

• DAPI
• FITC
• TRITC
• Cy5
• mCherry-LP

Hardware

• Nikon Ti Inverted Microscope
• Andor Clara Interline CCD (USB, Left port)
• In Vivo Scientific Cage incubator
• Oko CO2 Mixer
• Sola Light Engine Gen III 350-680nm (COM 4, Baud rate Auto)
• ASI MS2000 XY stage (COM3)

PC

• Windows 10 64-bit
• Xeon W3505, 2.3 GHz
• 6 GB RAM
• ATI FirePro 2260
• 500GB SSD

Firmware

• Ti-E V.5.1

Software

• Nikon Elements 5.02 build 1266
• Micro-Manager 2.0
• Micro-Manager 1.4