N-SIM (Decomissioned)

FPBASE

The N-SIM is no longer Operational! This page is being left up for historical information.

Optics

Objectives

1. Plan Apo I 10x/0.45
2. Plan Fluor ELWD 40x/0.65
3. Empty
4. Empty
5. Plan Apo IR 60x/1.27 Water
6. Apo TIRF 100x/1.49 Oil

Filter Turret 1

1. Empty
2. Empty
3. Analyzer
4. UV-2A
5. FITC
6. HYQ TRITC

Filter Turret 2

1. 488
2. 405
3. 561
4. Empty
5. H/M
6. 647

SIM Gratings

• 3D EX V-G 100X/1.49 (for 100x objective, 405nm - 561nm)
• 3D EX V-R 100x/1.49 (for 100x objective, 405nm - 640nm)

• 3D EX V-R 60x/1.27 - TIRF 488 100x/1.49 (for 60x objective, 405nm - 640nm or 100x objective, 488nm TIRF)

• TIRF561 100x/1.49
• 3D 1layer 100x/1.49
• 3D 1layer 60x/1.27 - TIRF488 100x/1.49

Hardware

• Nikon Ti-E Microscope
• Nikon motorized stage with piezo Z insert
• Andor DU897 on right port
• Nikon LUSU laser controller
• Nikon Intensilight

Interface Requirements

• USB for MCL nano drive
• USB for Nikon TI
• USB For Nikon AOTF
• Camera Card for DU897

Firmware

• Ti-E: v4.01
• HUB-AM: v3.20
• HUB-AS: v4.00
• Remote: v1.25
• Ti-JOY: v1.20

PC

• HP Z400
• Xeon W3670 (3.2GHz)
• 24 GB RAM
• 64 bit Windows 7
• 2 TB Hard drive
• NVidia Quadro Pro 600

Software

• NIS-Elements 5.02.00 (build 1266) Patch 02