UCSF CALM Microscopy Wiki
UCSF CALM Microscopy Wiki
Wiki
View the Project on GitHub
UCSF-CALM/wiki
Contents:
- Home
- Microscopes
- Spinning Disk Confocal
- TIRF/N-STORM
- Time Lapse
- 6D
- Crest LFOV/C2
- CSU-W1
- AZ100 Light Sheet
- OMX-SR
- TruLive3D Light Sheet
- C-Trap Optical Tweezers
- QLIPP Timelapse with Nanoindentor
- Snouty Light Sheet
- CVRI Nikon Spinning Disk
- CVRI Nikon Spinning Disk
- CVRI Leica SPE Confocal
- Other Equipment and Objectives
- Data Analysis Workstations
- Data Analysis
- Sample Preparation
- References & Education
- CALM Information
- Miscellaneous
Contents:
- Home
- Microscopes
- Spinning Disk Confocal
- TIRF/N-STORM
- Time Lapse
- 6D
- Crest LFOV/C2
- CSU-W1
- AZ100 Light Sheet
- OMX-SR
- TruLive3D Light Sheet
- C-Trap Optical Tweezers
- QLIPP Timelapse with Nanoindentor
- Snouty Light Sheet
- CVRI Nikon Spinning Disk
- CVRI Nikon Spinning Disk
- CVRI Leica SPE Confocal
- Other Equipment and Objectives
- Data Analysis Workstations
- Data Analysis
- Sample Preparation
- References & Education
- CALM Information
- Miscellaneous
Principles and Practices of Light Microscopy (Spring 2013)
[Microscopy References and
Education](Microscopy-References-and-Education.html)
Videotaped lectures
These are posted at this link as they are filmed. (UCSF login required)
Useful background reading
Mike Davidson’s Websites
Books
Lectures
Lectures are held in Rock Hall 102 from 10-11:30 am on Tuesdays and Thursdays. Schedule (subject to change; slides will be linked here as they become available):
Lecture 1 - April 2: What is light; the transmitted light microscope (Kurt Thorn)
📄 Lecture 1 - Microscopy I
Lecture 2 - April 4: Resolution and objective corrections (Kurt Thorn)
📄 Lecture 2 - Microscopy II
Lecture 3 - April 9: Brightfield contrasting; Phase / DIC (Orion Weiner)
📄 Lecture 3 - Phase Contrast and DIC
Lecture 4 - April 11: Fluorescence and fluorescence optics (Nico Stuurman)
📄 Lecture 4 - Fluorescence
Lecture 5 - April 16: Cameras, detectors, and light sources (Nico Stuurman)
📄 Lecture 5 - Detectors and Light Sources
Lecture 6 - April 18: PSF, resolution, and fourier optics (Kurt Thorn)
📄 Lecture 6 - Microscopy III
Lecture 7 - April 23: Immunofluorescence, small molecule dyes, Qdots, etc. (Kurt Thorn)
📄 Lecture 7 - Fluorescence and Small Molecule Probes
Lecture 8 - April 25: Fluorescent proteins (Kurt Thorn)
📄 Lecture 8 - Genetically Encoded Tags
Lecture 9 - April 30: Optical sectioning: Confocal (Kurt Thorn)
📄 Lecture 9 - Confocal
Lecture 10 - May 2: Optical sectioning: TIRF, 2p and SPIM (Kurt Thorn)
📄 Lecture 10 - TIRF, 2-Photon, and SPIM
Lecture 11 - May 7: Special techniques: FRAP, FRET, FLIM, etc. (Holly Aaron)
(No slides available)
Lecture 12 - May 9: Optical sectioning: Deconvolution (David Agard)
📄 Lecture 12 - Deconvolution
Lecture 13 - May 14: Super-resolution: STED and SIM (Bo Huang)
📄 Lecture 13 - Super-resolution by Patterned Illumination
Lecture 14 - May 16: Super-resolution: Localization Microscopy (Bo Huang)
📄 Lecture 14 - Super-resolution by Single-Molecule Switching
Lecture 15 - May 21: Fluorescent reporters and probes (Mark von Zastrow)
(No slides available)
Lecture 16 - May 23: Live Cell Imaging and Phototoxicity (Kurt Thorn)
📄 Lecture 16 - Live Cell Imaging
Lecture 17 - May 28: Optical Control of Living systems (Orion Weiner)
(No slides available)
Lecture 18 - May 30: Intravital microscopy and 2 photon (Max Krummel)
(No slides available)
Lecture 19 - June 4: Image Analysis (Nico Stuurman)
(No slides available)
Lecture 20 - June 6: Electron Microscopy (Yifan Cheng)
(No slides available)
Image Analysis Lab
For the image analysis lab, you will need to download and install the Fiji distribution of ImageJ. Fiji is free and available for both Macs and PCs. Download the manual and files below.