Fluorescent Proteins for Localization Microscopy

STORM sample preparation and imaging

Protein	λex	λem	Contrast Ratio	Nphotons (median)	Notes	Reference
PS-CFP2	490	511	2000	260	Cyan-to-green photoswitchable	6, Evrogen
mGeos-M	503	514	—	387	Reversibly photoswitchable	7
PATagRFP	562	596	540	500?	Photoactivatable	8, Evrogen
PAmCherry	564	595	4000	348	Photoactivatable	1
tdEos	569	581	—	499	Green-to-red photoswitchable	3, 4, 5
mEos2	573	584	—	379	Green-to-red photoswitchable	3, 4
mEos3.2	572	580	—	482	Green-to-red photoswitchable	3
PSmOrange	631	662	10700	337	Orange-to-far-red photoswitchable	2

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1. Subach, FV. et al., Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Nat. Meth. 2009, 6: p. 153-159.

2. Subach, OM. et al., A photoswitchable orange-to-far-red fluorescent protein, PSmOrange. Nat. Meth. 2011, 8: p. 771-777.

3. Zhang, M. et al., Rational design of true monomeric and bright photoactivatable fluorescent proteins. Nat. Meth. 2012, 9: p. 727-729.

4. McKinney, SA. et al., A bright and photostable photoconvertible fluorescent protein. Nat Meth. 2009, 6: p. 131-133.

5. Wiedenmann, J. et al., EosFP, a fluorescent marker protein with UV-inducible green-to-red fluorescence conversion. Proc Natl Acad Sci 2004. 101(45): p. 15905-15910.

6. Shroff, H. et al., Dual-color superresolution imaging of genetically expressed probes within individual adhesion complexes. Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20308-13.

7. Chang, H. et al., A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):4455-60.

8. Subach, FV. et al., Bright monomeric photoactivatable red fluorescent protein for two-color super-resolution sptPALM of live cells. J Am Chem Soc. 2010 May 12;132(18):6481-91.