UCSF CALM Microscopy Wiki

UCSF CALM Microscopy Wiki

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Make your own flow chamber

Did you know you can make your own flow chamber slide with some double-sided tape?

Here is an example of making a simple flow chamber with double-side tape https://www.youtube.com/watch?app=desktop&v=Y_7YaVIK4Uc

We also have a Silhouette (automatic cutter) and double sided 3M Sticky paper to cut more precise/consistent flow chambers in the CALM. Ask us!S

Example Chambers in papers!

Chamber From : https://www.researchgate.net/figure/Sketch-of-the-flow-chamber-A-glass-coverslip-smeared-with-nitrocellulose-is-attached_fig1_335370438

This chamber is a good example of one that allows you to add while imaging!

Sketch of the flow chamber. A glass coverslip, smeared with nitrocellulose, is attached onto a microscope slide through double sticky tape stripes to form a flow-cell of about 20 ml volume. Solutions are flown from one side of the chamber with a pipette and sucked from the other side through a filter paper to create a flow in the arrow direction.

Chamber from: https://www.sciencedirect.com/science/article/pii/S0076687919302587#f0020

Fig. 3

Chamber from:  https://www.jove.com/t/63952/self-assembly-of-microtubule-tactoids

Figure 3

Chamber from: https://www.researchgate.net/publication/51506573_Reconstitution_and_Quantification_of_Dynamic_Microtubule_End_Tracking_In_Vitro_Using_TIRF_Microscopy?_tp=eyJjb250ZXh0Ijp7ImZpcnN0UGFnZSI6Il9kaXJlY3QiLCJwYWdlIjoiX2RpcmVjdCJ9fQ

Scheme of the flow cell and the setup. (a) Scheme of the flow chamber. Two strips of double-sided tape are placed at a distance of 5 mm between one ethanol vapor-cleaned and PLL-PEG-treated glass and one biotin-PEG-functionalized glass coverslip. (b) Cartoon of the microtubule end-tracking assay. A biotinylated and GMP-CPP-stabilized microtubule seed is immobilized via NeutrAvidin on functionalized glass and allowed to grow dynamically in the presence of free nonbiotinylated tubulin and GTP in the solution. Imaging is performed with dual-color, time-lapse TIRF microscopy.